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| Souza,Marcos Michel de; Manzine,Livia Regina; Silva,Marcos Vinicius G. da; Bettini,Jefferson; Portugal,Rodrigo Vilares; Cruz,Angela Kaysel; Arruda,Eurico; Thiemann,Otavio Henrique. |
| Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania guyanensis; Leishmania RNA virus; LRV; Virus isolation; Electron microscopy. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044 |
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| Anibal,Paula Cristina; Peixoto,Iza Teixeira Alves; Foglio,Mary Ann; Höfling,José Francisco. |
| Ethanolic crude extracts prepared from the arils and seeds, pericarp, peels and from the whole fruit of Punica granatum, known as pomegranate, had their antifungal activity tested against Candida spp. The ethanolic crude extracts were analyzed by Mass Spectrometry and yielded many compounds such as punicalagin and galladydilacton. The extracts from the pericarp and peel showed activity against Candida spp., with MICs of 125 µg/mL. The effect of pericarp and peel extracts upon the morphological and structure of C. albicans and C. krusei were examined by scanning and transmission electron microscopy, with the visualization of an irregular membrane and hyphae, formation of vacuoles and thickening of the cell wall. The data obtained revealed potential... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Punica granatum; Punicalagin; Antifungal activity; Candida spp.; Electron microscopy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300027 |
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| Bjerling,P.; Ekwall,K.. |
| Centromere function requires the proper coordination of several subfunctions, such as kinetochore assembly, sister chromatid cohesion, binding of kinetochore microtubules, orientation of sister kinetochores to opposite spindle poles, and their movement towards the spindle poles. Centromere structure appears to be organized in different, separable domains in order to accomplish these functions. Despite the conserved nature of centromere functions, the molecular genetic definition of the DNA sequences that form a centromere in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, in the fruit fly Drosophila melanogaster, and in humans has revealed little conservation at the level of centromere DNA sequences. Also at the protein level few... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Centromere; Kinetochore; Histone acetylation; Electron microscopy. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500001 |
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| Castejón,Orlando J.. |
| The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerebellar cells; Synaptic contacts; Electron microscopy; Immunohistochemistry. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100001 |
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| ARNEODO,JOEL D.; LORENZO,EUGENIA; LAGUNA,IRMA G.; ABDALA,GUILLERMINA; TRUOL,GRACIELA A.. |
| The Mal de Río Cuarto disease is caused by Mal de Río Cuarto virus (MRCV) transmitted by Delphacodes kuscheli. Comparative studies were carried out on the cytopathological alterations produced by MRCV in corn (Zea mays), wheat (Triticum aestivum) and barley (Hordeum vulgare), as seen with a transmission electron microscope. Corn plants were infected with viruliferous D. kuscheli collected from the endemic disease area (i.e. Río Cuarto County, Córdoba, Argentina). For the viral transmission to small grain cereal plants, laboratory rared insects were used. In this case, the inoculum source was wheat and barley plants infected with MRCV isolate grown in a greenhouse. Leaf samples with conspicuous symptoms were collected: enations and size reduction in corn;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Virus characterization; Electron microscopy; Fijivirus; Reoviridae; Cereals. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300010 |
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| NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B.. |
| An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007 |
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| Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.. |
| The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,... |
| Tipo: Text |
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf |
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| Cen Caamal, Julio César. |
| En los últimos años la superficie cultivada de Agave tequilana Weber var. Azul en el estado de Jalisco ha disminuido, debido a la presencia de una enfermedad cuyos síntomas se manifiestan como pudrición blanda del cogollo y muerte de la planta. El objetivo principal fue determinar el agente causal de esta enfermedad. Para ello se realizaron aislamientos, pruebas bioquímicas y moleculares mediante la amplificación del fragmento 16S DNAr y pruebas de patogenicidad con resultados positivos. El patógeno purificado fue una bacteria Gram positiva; en microscopia electrónica se observaron células en forma de bacilo, con presencia de endosporas en la parte terminal y flagelos peritricos. Creció hasta 50°C en medio de agar nutritivo (AN). Este organismo, se... |
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Palavras-chave: Patogenicidad; Prueba molecular; Microscopia electrónica; Pathogenicity; Molecular testing; Electron microscopy; Fitopatología; Maestría. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1747 |
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| Nascimento,R.S.; Santiago,M.F.; Marques,S.A.; Allodi,S.; Martinez,A.M.B.. |
| Peripheral glial cells consist of satellite, enteric glial, and Schwann cells. In dorsal root ganglia, besides pseudo-unipolar neurons, myelinated and nonmyelinated fibers, macrophages, and fibroblasts, satellite cells also constitute the resident components. Information on satellite cells is not abundant; however, they appear to provide mechanical and metabolic support for neurons by forming an envelope surrounding their cell bodies. Although there is a heterogeneous population of neurons in the dorsal root ganglia, satellite cells have been described to be a homogeneous group of perineuronal cells. Our objective was to characterize the ultrastructure, immunohistochemistry, and histochemistry of the satellite cells of the dorsal root ganglia of 17 adult... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Satellite glial cells; Peripheral nervous system; Vimentin; S-100; Electron microscopy. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100011 |
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| Take,G.; Erdogan,D.; Helvacioglu,F.; Göktas,G.; Ozbey,G.; Uluoglu,C.; Yücel,B.; Guney,Y.; Hicsonmez,A.; Ozkan,S.. |
| The effect of ionizing irradiation on testes and the protective effects of melatonin were investigated by immunohistochemical and electron microscopic methods. Eighty-two adult male Wistar rats were divided into 10 groups. The rats in the irradiated groups were exposed to a sublethal irradiation dose of 8 Gy, either to the total body or abdominopelvic region using a 60Co source at a focus of 80 cm away from the skin in the morning or evening together with vehicle (20% ethanol) or melatonin administered 24 h before (10 mg/kg), immediately before (20 mg/kg) and 24 h after irradiation (10 mg/kg), all ip. Caspace-3 immunoreactivity was increased in the irradiated group compared to control (P < 0.05). Melatonin-treated groups showed less apoptosis as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Testes; Gamma-irradiation; Melatonin; Immunohistochemistry; Electron microscopy. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700006 |
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| Allodi,S.; Taffarel,M.. |
| Invertebrate glial cells show a variety of morphologies depending on species and location. They have been classified according to relatively general morphological or functional criteria and also to their location. The present study was carried out to characterize the organization of glial cells and their processes in the zona fasciculata and in the protocerebral tract of the crab Ucides cordatus. We performed routine and cytochemical procedures for electron microscopy analysis. Semithin sections were observed at the light microscope. The Thiéry procedure indicated the presence of carbohydrates, particularly glycogen, in tissue and in cells. To better visualize the axonal ensheathment at the ultrastructural level, we employed a method to enhance the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Glial cells; Crustaceans; Electron microscopy; Cytochemistry; Ucides cordatus. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300013 |
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| Teixeira,Glauco Antonio; Alves,Eduardo; Amaral,Douglas Carvalho; Machado,José da Cruz; Perina,Fabiano José. |
| Stem and ear rot caused by Stenocarpella maydis are responsible for severe losses in maize production. Treatment of seeds with fungicides may induce environmental damage. Hence, this study aimed to evaluate the effects of essential oils extracted from Cymbopogon winterianus, Thymus vulgaris, Cymbopogon citratus, Corymbia citriodora, Cinnamomum zeylanicum, and Syzygium aromaticum on the development of in vitro S. maydis. In addition, maize seeds were treated with these essential oils to determine their possible mode of action and effects. The oils from S. aromaticum, C. zeylanicum, and T. vulgaris inhibited fungal development at concentrations higher than 0.025%. The oils from S. aromaticum and C. zeylanicum showed seed germination rates of 89.0% and 84.5%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenocarpella maydis; Zea mays; Alternative control; Electron microscopy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100004 |
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| Registros recuperados: 50 | |
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